FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979749
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46780.23629258052409674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37600.043.801691
TGGTATC38200.042.88262
GTATCAA148800.027.7720471
AGAGTAC88950.026.96008111
CAGAGTA92800.025.98910310
TATCAAC163850.025.1954252
CGTCACG550.00122530725.00545939
ATCAACG174550.023.6176513
GGTATCA71750.023.5952243
TCAACGC174700.023.502394
CAACGCA178450.023.0393035
CGTCGGA600.002033462322.9216740
AGTACGG104250.022.79230913
AACGCAG181800.022.7682326
GAGTACG105600.022.55301712
TACGGGG41600.020.89028515
GTACGGG114800.020.62585314
ACGCAGA207150.019.9436747
CGCAGAG206950.019.8617128
ACGGGAT22800.019.7820216
Produced by FastQC (version 0.11.5)