FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620014
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.5462779872712552No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.5428909669781651No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.3401536094346257No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.2903160251220134No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16140.260316702526072No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.24402674778311456No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.19676975036047573No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11250.18144751570125836No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10450.1685445812513911No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10360.16709300112578104No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8510.137254965210463No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.12483589080246577No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA7660.12354559735747904No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA7500.12096501046750557No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG7460.12031986374501222No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT7270.11725541681316873No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.1170941301325454No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC7030.11338453647820855No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6970.11241681639446852No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA6850.11048137622698842No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.1072556426145216No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA6270.10112674875083466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23000.048.596952
GTGGTAT22950.047.9814451
ATACGGG853.8198777E-1045.017341
AAGTACG2000.044.6421971
GTATCAA72950.037.125341
TATCAAC82900.034.4505272
GACAGGT2700.033.0682951
GTCGAGG14950.031.5546158
TCGAGGT14750.031.4062149
ATCAACG91850.031.0473443
TCAACGC92500.030.8751224
AACGCAG92400.030.770556
CAACGCA94950.030.0784475
ATATCAA5250.029.1540891
GGTATCA40600.028.995783
GCAGCGT1755.511538E-1026.7245791
AGTCGAG22600.026.7030587
CGTCAGA1606.2354957E-926.5620215
ACGCAGA109150.026.0874827
CGCAGAG109600.025.9803718
Produced by FastQC (version 0.11.5)