FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620014
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13410.21628543871589997No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9480.15289977323092704No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT9400.15160947978594033No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7270.11725541681316873No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA6710.10822336269826166No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT6450.10402990900205478No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC6410.10338476227956142No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA6390.10306218891831474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTC300.001930423536.6454287
TGGTATC27250.036.410092
GTGGTAT27600.035.965771
GACAGGT2100.028.8067761
AGAGTAC55250.025.08965511
CAGAGTA55900.024.94552210
GTATCAA91650.024.9021531
TATCAAC100600.022.8123262
CGGGATC2900.022.76196117
GGGATCG2300.021.52489718
GGTATCA48750.021.3107263
TCGACAC2200.021.25311722
GTAGCGA650.00323987721.151839
GTCGAGC650.003245946321.1450068
AACGCAG107900.021.1415926
ATCAACG108450.021.059723
GAGTACG65450.021.01148812
TCAACGC109300.020.9462324
AGTACGG65150.020.89715613
CGGGGAA5800.020.86513117
Produced by FastQC (version 0.11.5)