FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360225
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA50300.3697917623922513No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA42290.3109044459556324No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT41880.30789023874726607No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC35820.26333878586263304No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT28850.21209726332040657No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC28380.208641952618133No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA28050.20621588340164312No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA27790.204304434928045No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC27300.2007020897278024No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC27150.19959933099303423No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG25510.1875425021595692No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA25460.18717491591464647No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT25390.18666029517175467No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT22970.16886912091749529No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG20910.1537245676266794No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG20480.15056332592034405No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT20410.15004870517745225No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT20390.14990167067948318No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG20040.14732856696502417No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG19830.14578470473634877No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG19570.14387325626275066No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA18310.13461008289069823No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC17760.13056663419654838No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA17270.12696428899630574No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG17220.12659670275138304No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG17190.12637615100442942No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA16490.12122994357551141No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT16420.1207153228326196No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.12034773658769687No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG16230.1193184951019133No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG16200.11909794335495966No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA16080.11821573636714514No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT15800.11615725339557793No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT15120.11115808046462902No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG14890.10946718373798453No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA14880.10939366648899998No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC14660.10777628701134002No Hit
ATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTC14620.10748221801540186No Hit
ATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCG14380.10571780403977284No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC14300.10512966604789649No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT13810.1015273208476539No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13760.10115973460273116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62250.048.8242761
GGTATCA28900.045.449571
TGGTATC12850.042.3315542
TATCAAC73600.041.2266242
TCAACGC76850.039.424954
GTGGTAT14250.039.3756941
ATCAACG78000.038.843683
CAACGCA78550.038.62585
AACGCAG79850.037.996956
CGCAGAG83100.036.458438
ACGCAGA83650.036.2695167
GCAGAGT90650.033.468789
CAGAGTA77400.026.84586510-11
TATACTG1701.19325705E-824.9963465
AGAGTAC75700.023.4072110-11
GAGTACG88050.023.40571212-13
ACGGGTC8050.021.9059316-17
TACTGGT7750.021.3855442
AGTACGG85450.020.51263212-13
TACGGGT16150.020.39100514-15
Produced by FastQC (version 0.11.5)