FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976492
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.5313919622485387No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.3639558747025065No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.27117477664947587No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22540.23082626380963694No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16180.16569516186512537No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT14820.15176775641787132No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14640.14992442334397005No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13760.14091257276045271No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13240.13558738832473793No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12920.13231035174891345No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA12310.12606350077624803No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA12040.12329850116539612No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC11920.12206961244946195No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA11000.11264813229396656No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT10950.11213609532899399No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC10940.11203368793599948No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG10880.11141924357803239No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC9840.10076887470660283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15350.049.834582
GTGGTAT15500.049.085671
GTATCAA53950.040.3377721
GGTATCA29750.039.146871
TATCAAC62200.035.0504572
TCAACGC66000.033.2882584
ATCAACG65950.033.1846243
CAACGCA66400.033.0877275
AACGCAG67600.032.5632326
ACGCAGA75150.029.4033347
CGCAGAG75300.029.1754658
GCAGAGT79550.027.6701689
CAGAGTA46450.026.98724210-11
ACCGGGC1201.2774675E-524.7878598
AGCGTCA1901.4406396E-924.6027413
GACCTAG1251.7490147E-523.8024371
GAGTACG53750.023.12435312-13
GACAGGT3800.022.3707121
AGAGTAC45200.021.9048210-11
GTACTGG4750.021.4758831
Produced by FastQC (version 0.11.5)