FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976492
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29950.3067101420185726No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18980.1943692319035896No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14220.1456233128382004No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC14200.14541849805221138No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11960.12247924202144No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT11840.12125035330550583No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC11610.11889498326663199No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA11320.11592516886979105No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA11090.11356979883091721No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC10700.10957591050413111No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA10560.10814220700220792No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG10470.10722054046525727No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT10090.1033290595314657No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA9930.10169054124355345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9450.032.287422
GTGGTAT9750.031.0279241
GGTATCA22250.030.5303361
GTATCAA48550.029.2863981
TATCAAC56150.025.5052852
GCTATAC651.0928095E-425.3708823
AGAGTAC56650.024.17529711
ATCAACG58700.024.1138653
AACGCAG59100.024.0473656
TCAACGC58800.023.9805974
CAACGCA59450.023.9495755
CAGAGTA57450.023.73926510
ACGCAGA61450.023.1724687
CGCAGAG61600.023.1606668
GCAGAGT64500.022.1227349
AGTACGG62800.021.24071113
CGGGATT3250.021.15648717
GAGTACG63600.021.14542212
ACGGGAT8950.020.89646516
GATCGAC2001.8189894E-1220.62651820
Produced by FastQC (version 0.11.5)