FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495021
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT60060.40173348735569603No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.2635414485816587No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.1839439044668938No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.11872742924681325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12500.046.923062
GTGGTAT13500.043.454551
GTATCAA50500.042.5940631
TATCAAC56300.037.897682
TCAACGC59600.035.938344
CAACGCA59900.035.7583475
GGTATCA25650.035.632071
ATCAACG60650.035.386233
AACGCAG62600.034.488766
ACGCAGA69550.031.1034727
CGCAGAG70200.030.5733138
GCAGAGT74500.028.9798229
CAGAGTA49600.024.54885310-11
GAGTACG54950.021.84900912-13
AGAGTAC46850.021.63550410-11
GTACGGG61600.019.24787114-15
AGTACGG52850.018.97789412-13
GACAGGT7500.017.5707531
ATTACCG1752.3294658E-416.9994055
AGAGTCG25400.016.7316975
Produced by FastQC (version 0.11.5)