FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495021
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117300.7846043634169688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10950.032.1679151
TGGTATC11050.031.1078192
GTATCAA48600.029.2739521
TATAGCG507.0463284E-427.4910375
TATCAAC53500.026.6769052
GGTATCA21850.026.1962411
ATCAACG55250.025.5787933
TCAACGC55700.025.3695964
CAACGCA56500.025.0095465
AACGCAG57250.024.586696
AGAGTAC53700.024.32353411
CAGAGTA55750.023.67495510
ACGCAGA59650.023.5974547
CGCAGAG60350.023.2781938
CGGGATA2400.022.91686217
GCAGAGT64500.021.7840929
AGTACGG61250.021.1022213
ACGGGAT10600.021.0143316
GAGTACG63050.020.58632312
TCGACAC3200.018.9057822
Produced by FastQC (version 0.11.5)