FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818803
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24380.2977517180567243No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13690.16719528384727464No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT11240.1372735566430509No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC10450.12762532623842365No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA8940.1091837719207184No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA8790.10735182943882716No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT8550.10442072146780115No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT8380.10234451998832443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8250.032.662312
GTGGTAT8900.030.9079781
GTATCAA42250.029.3636761
TACGAGG507.0231047E-427.50473615
ACCGTCC400.00788870427.4879388
GGTATCA18750.027.4347441
TATCAAC50100.025.0814862
AGAGTAC45950.024.59561311
ATCAACG51600.024.2886983
TCAACGC51650.024.2651844
AACGCAG51650.024.0552716
CAGAGTA47350.024.03380610
CAACGCA52800.023.8928435
TTAACGG701.7979063E-423.57548735
CGCAGAG54550.022.8268288
ACGCAGA55000.022.6900427
TAACGGC752.868336E-422.00378836
TACGGGG18200.021.91311515
AGTACGG50050.021.7593313
GCAGAGT57200.021.7212379
Produced by FastQC (version 0.11.5)