FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951440
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36550.38415454469015387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8700.026.5636861
GTATCAA33800.026.3728221
AACGCAG37800.024.2167196
AGAGTAC34750.024.21272711
CAGAGTA35000.024.19181310
TATCAAC38200.023.681522
ACGCAGA38750.023.623027
ATCAACG38450.023.5201243
CGCAGAG38600.023.5011738
TCAACGC38500.023.4883444
CAACGCA38800.023.3775735
TGGTATC9950.022.936762
TGCCGAC4700.022.2405555
GCAGAGT41950.021.7554979
GGTATCA16750.021.6814061
AGTACGG39400.021.35962513
GAGTACG41050.020.49784312
CGGGATA1301.6337708E-619.04002817
ATTAGAG1301.6420163E-619.030023
GACAGGT3950.018.8059391
Produced by FastQC (version 0.11.5)