FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829518
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93851.1313799097789319No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG257.87394E-443.9787257
GTGGTAT8900.040.4799271
TGGTATC9650.036.7483182
CCGATCG553.4897086E-529.99996646
GTATCAA40750.028.3452381
TCGAACG400.00787193127.4999720
GGTCGTC400.00789048727.4867047
GGTATCA19000.025.909431
TATCAAC45400.025.6129472
TCAACGC48400.023.9031244
ATCAACG48950.023.6373983
CAACGCA49450.023.5651325
AACGCAG50600.023.1953036
AGAGTAC43600.023.01759711
CAGAGTA44050.022.96832710
ACGCAGA51600.022.692517
CGCAGAG51200.022.5490478
TCGACGT1658.367351E-1121.66925815
GCAGAGT53200.021.5489279
GTATAGC650.00323788621.1550981
Produced by FastQC (version 0.11.5)