FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987729
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13240.13404486453268052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22300.037.58692
GTGGTAT23500.035.917821
TCGGCCG350.00409553231.43456325
GTATCAA71350.025.6637271
AGAGTAC51100.023.19561211
TATCAAC79100.023.0344892
CGTACGA600.002034270422.91871535
CAGAGTA51900.022.78508210
ATACCGC852.580162E-522.635326
GGTATCA38100.022.575583
ATCAACG85850.021.1903063
TCAACGC86000.021.089444
AACGCAG87350.020.9565056
GCTTGTA3850.020.709841
CAACGCA88300.020.5712325
GTCTAAG1108.264624E-619.9957081
ACGTACG700.004972975219.64461334
TCGCCCC1301.633789E-619.04016338
ACGCAGA98200.018.698927
CGCAGAG98800.018.5900698
Produced by FastQC (version 0.11.5)