FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816858
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.30552745453965036No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA38640.21267484855723454No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.20320795571255432No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC26580.1462965184951163No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT26330.14492051662815694No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25640.1411227514753492No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG22910.1260968110881533No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA21230.11685007854218656No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA20530.11299727331470044No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA19290.10617230405458214No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC19000.10457614188890932No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG18940.10424590144083909No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC18940.10424590144083909No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT18930.10419086136616071No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA18800.10347534039534184No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG18340.10094349696013667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18200.046.9515721
TGGTATC18150.046.5970572
GTATCAA65550.044.232061
TATCAAC76800.037.850962
TCAACGC79650.036.6536454
ATCAACG80350.036.3343163
CAACGCA80300.036.25115
AACGCAG82900.035.1654176
GGTATCA34050.034.4601751
ACGCAGA90800.032.104997
CGCAGAG91950.031.6572428
GCAGAGT98950.029.5465569
CAGAGTA64250.025.4974910-11
AGAGTAC62000.023.13280310-11
GAGTACG73050.022.19323212-13
AGTACGG70850.020.12328312-13
GTACGGG86050.018.69274714-15
GACAGGT10200.017.92231
TAAGTGC21600.017.706596
GTTAGAC2708.228744E-817.313113
Produced by FastQC (version 0.11.5)