FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816858
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT33960.1869160936077558No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC25520.1404622705792087No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT25360.13958162938435473No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC21610.11894160137996476No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA20170.11101583062627898No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA19370.10661262465200913No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT18930.10419086136616071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59350.030.966941
TGGTATC12650.030.6484132
GTGGTAT13800.029.307641
GTCGCGT400.00787162227.50192814
TATCAAC68900.026.8580992
GGTATCA27500.026.512811
TCAACGC71700.025.915024
ATCAACG71750.025.8224743
CAACGCA73050.025.5859035
AACGCAG74150.025.2461916
AGAGTAC70550.024.28188511
ACGCAGA76950.024.222387
CAGAGTA71900.023.86158410
CGCAGAG78050.023.634448
GCAGAGT82800.022.2128099
AGTACGG79050.021.36021813
GAGTACG80750.020.90995612
CGGGATA2300.020.3275117
ACGGGAT11150.020.22563416
TTAACGG2250.019.55746535
Produced by FastQC (version 0.11.5)