FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571780
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.34278918465143937No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.30851026618629546No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11150.1950050718807933No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10990.19220679282241424No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.18905872888173775No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9860.17244394697261184No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.17156948476686837No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9050.15827765923956766No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8680.151806638917066No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA8480.14830879009409215No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG8460.14795900521179473No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA8070.1411382000069957No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.13764035118402182No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.12819615936199236No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC6970.12190003148063941No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG6410.11210605477631258No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA6230.10895799083563609No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC6160.10773374374759521No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT5850.10231207807198572No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG5750.1005631536604988No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCA250.007792611651.0027289
TGGTATC13150.041.6905982
GTATCAA49300.039.491781
TATCAAC53350.036.802872
AACGCAG55650.035.2054566
GTGGTAT15450.034.9432561
TCAACGC57000.034.4461984
ATCAACG57800.034.0429653
GTCGAGG7300.033.1838048
CAACGCA59200.033.0943155
ACGCAGA61900.031.5821257
CGCAGAG62050.031.5742688
GCAGAGT67350.029.029029
TCGAGGT8050.027.4548859
GCTTGTA3900.027.2498151
GGTATCA26900.027.180931
TCGAGGG957.440768E-526.8435429
GTCAGAT1851.0550139E-925.2694116
GATCGAC1551.3453973E-724.6765827
CAGAGTA39500.024.64055610-11
Produced by FastQC (version 0.11.5)