FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571780
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA10320.18048899926545175No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9350.16352443247402848No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT9040.15810276679841898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8870.15512959529889117No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8850.1547798104165938No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7730.13519185700794012No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA7640.13361782503760186No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC7390.12924551400888454No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT7210.12609745006820805No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA6420.11228094721746126No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA6260.10948266815908217No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG6160.10773374374759521No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT5860.10248697051313442No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA5830.10196229318968834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCTC401.9005298E-434.3744541
TGGTATC16400.034.0301552
GTGGTAT17950.031.2529811
GGCCGGG400.00786712527.5019755
CGGACTA805.1764255E-727.4971569
TACTATA400.00788057427.4923462
CGGGCCA400.00788730627.4875354
AGAGTAC42350.025.97133811
CCGGACT858.7950866E-725.8774138
GTATCAA56500.025.796051
CAGAGTA43200.025.33302910
AGGCCTA550.001225913424.99960133
CGAAGAG550.00122716524.9952268
GATCGGC1001.3075078E-724.74960517
TATCAAC62250.023.5837942
AGTCCGG1052.0914558E-723.5751854
GACAGGT2450.023.5710541
CGATCGG1052.0950029E-723.57105416
TCCGGAC952.321729E-623.1473967
GGCCTAC600.002032403622.92031334
Produced by FastQC (version 0.11.5)