FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865972
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.3199872513199041No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.29642990766445104No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.19203854166185513No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.16432401971426328No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12700.14665601197267347No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.1333761368727857No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.13095111620237143No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA11320.13072016185280816No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10740.12402248571547349No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC9990.11536169760685101No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9880.11409144868425307No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9340.1078556812460449No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA9120.10531518340084899No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG9100.10508422905128573No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA9060.1046223203521592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAC805.413185E-737.1852764
TGGTATC24750.034.85652
GTATCAA71650.034.0037731
GACAGGT3150.033.7456781
GTGGTAT26100.033.2336771
AACGCAG78350.031.8934366
GTCGAGG9600.029.6596858
TATCAAC84800.029.5176932
TCAACGC85800.029.1736644
ATCAACG87050.028.7547423
CAACGCA88250.028.41195
GCCGGAC300.00680659128.33327558-59
ACGCAGA88550.028.267657
CGCAGAG89550.027.904538
ACAGGTA4450.026.753881
GCAGAGT95050.026.2451449
GGTATCA43500.025.7072241
CAGGTAT5050.024.417171
CGGGAGC1751.6207196E-824.2842626
CAGAGTA56400.022.75770210-11
Produced by FastQC (version 0.11.5)