FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706564
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11100.15709829541272977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15150.039.179162
GTGGTAT15450.038.6290741
GTATCAA50700.025.9842741
TTAAACG759.7206685E-625.67509722
CCGCTCG550.001223740325.00821322
AGAGTAC34650.024.6077711
CAGAGTA35250.024.4212710
TATCAAC56750.023.1944272
TTGTACG600.00204345822.8998553
GGTATCA26850.022.6184063
GCTTGTA2100.022.2644861
ATCAACG58650.022.2556133
AACGCAG58850.022.2266756
TCAACGC58800.022.198844
CAACGCA59600.021.900875
ACGGGAT6450.020.46893716
AGTACGG42650.019.8630313
ACGCAGA66300.019.7291077
CGCAGAG66550.019.5779518
GAGTACG43550.019.51570112
Produced by FastQC (version 0.11.5)