FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301332
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.24690374736171397No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.2309744733383776No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.13606254894933165No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.12511117305828787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGCT200.00322159863.7478837
TGGTATC3800.059.274352
GTGGTAT3750.058.9607351
AGCGTCA401.45595E-553.1232383
GTATCAA14200.038.3276671
GGTATCA7800.037.068511
CAGCGTC601.5930519E-435.4154932
TATCAAC16600.033.5380442
ATCAACG17800.031.5158063
TCAACGC18150.030.9080644
CAACGCA18350.030.5711965
AACGCAG19750.028.8344866
ACAGGTA1203.9570295E-728.3465061
ACAGACG755.879441E-428.3465061
GCAGCGT755.879441E-428.3465061
GCGTCAG755.8965984E-428.3323964
TCGAGGT2700.028.3323929
ATAGGGT808.5983105E-426.5616198
GTCGAGG3100.026.0475228
ACGCAGA22150.025.7102097
Produced by FastQC (version 0.11.5)