FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955859
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46610.48762422072711564No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.3428329910583046No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT28580.2989980739837152No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21300.22283621329087241No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.10158402023729442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10950.054.7311482
GTGGTAT11750.052.827271
GTATCAA41200.043.547021
TATCAAC46950.038.6564372
TCAACGC50300.035.9053884
ATCAACG50550.035.7278183
CAACGCA51150.035.308725
GGTATCA20500.034.0120771
TATACCG500.00279033533.9960675
AACGCAG53750.033.600766
ACGCAGA59250.030.4099837
ATACCGT853.4929937E-529.9965296
CGCAGAG60000.029.9590328
GCAGAGT62450.028.8517469
CCTATAC1353.534842E-828.3300573
GCCTAAC850.00122386725.008881
GTATTAG3100.024.6861861
CAGAGTA36550.024.64831210-11
ATAGTAC2053.4724508E-922.8022383
AGAGTAC34700.022.53341710-11
Produced by FastQC (version 0.11.5)