FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955859
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57020.5965314968002603No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG300.001930495336.6471675
TGGTATC8550.034.4095082
GTGGTAT9250.032.1196021
GTATCAA37200.029.2107221
ACCGCGT400.00789092327.4868168
TATCAAC41600.026.2396472
AGAGTAC39300.025.2539911
TCAACGC43450.024.8601914
CAACGCA43950.024.827525
ATCAACG43750.024.6281873
CAGAGTA41100.024.47987210
GGTATCA17600.024.0711141
AACGCAG45350.023.879256
ACGCAGA46500.023.2886857
CGCAGAG47000.023.0421418
CGCCGTT600.002034142822.91886917
AGTACGG44500.021.87850613
GCAGAGT50300.021.5873349
GAGTACG45350.021.46169512
TACGGGC5350.020.56272315
Produced by FastQC (version 0.11.5)