FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005413708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921256
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT118771.2892181977647905No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.4970388252559549No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.3796990195993296No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.2584515053361932No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT20330.22067698880658576RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT14030.15229208819264134RNA PCR Primer, Index 29 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC13630.14795018974096236No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.10767908160163951No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)