FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862437
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46380.5377784116405024No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT35530.41197212086216156No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.4022322789954512No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.27167201778216843No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.10574685455285429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11200.055.8033681
TGGTATC11500.054.331872
GTATCAA42450.041.6655121
TATCAAC48700.036.8314322
GGTATCA21800.036.665911
ATCAACG52000.034.404333
TCAACGC52200.034.272524
CAACGCA53050.033.7233855
AACGCAG56050.031.99426
CGCAGAG60350.029.714588
ACGCAGA61000.029.4676117
GCAGAGT62500.028.6923989
CAGAGTA34600.025.60807810-11
AGCGTCA1751.621811E-824.2826673
GAGTACG37950.023.40557512-13
AGAGTAC33650.023.30012910-11
AGTACGG36950.021.10602412-13
GTACGGG41850.021.02130514-15
TATTAGG2703.9872248E-918.89092
ACGGGAA6300.018.55140916-17
Produced by FastQC (version 0.11.5)