FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862437
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71430.8282344101656122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7000.040.4758952
GTGGTAT7150.040.0370251
GTATCAA34950.028.3524021
GGTATCA16600.027.028011
ACGGGGT5350.025.18829716
TATCAAC39850.025.1264042
TCAACGC40700.024.1806964
AGAGTAC37700.024.0687511
CAGAGTA38250.023.93832410
ATCAACG41000.023.873823
CAACGCA42100.023.703085
AACGCAG42950.023.3619966
TACGGGG17400.022.75986915
CGCAGAG44000.022.618378
ACGCAGA45050.022.3352977
AGTACGG41150.021.65242413
GAGTACG42100.021.35731312
GCAGAGT47000.021.0588769
GTACGGG45050.019.77910814
CGGGGTT2650.018.6802717
Produced by FastQC (version 0.11.5)