FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGTTGTCTTTTTTTCCTATGTCTTTTGGCTCAAGCAACATGTAT11.0204081632653061No Hit
CTCTAGTATGGAGGCTCATAATTCCTTTTAACGTCTAGGGCATGACCAAT11.0204081632653061No Hit
CCTATACTTCAGCTTGACATTGAACTTTGATCTTAATTGTGTAGGATAGG11.0204081632653061No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTT11.0204081632653061No Hit
TATCAACGCAGAGTACGGGATGCTATGTGTGCGTGTTATTATTAATAGGT11.0204081632653061No Hit
ACATTAACCCTGTATGCTGCATCCTTGCTATAACTGCTTGTTAGTTCACA11.0204081632653061No Hit
GAAGTGAAATATGGAATGACAGTAACCAAGTGATAAAGTTATCATAAGAA11.0204081632653061No Hit
CTTATGTGCAGGCTGGTTCCCAAAGACTTGCAAGAAATGTTGAAAGACAT11.0204081632653061No Hit
CAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCTATGGAGCAATC11.0204081632653061No Hit
ATTTCTGGAATTTCACATGACAAAATTAAACTATTAGAAGCAGATAATAT11.0204081632653061No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG11.0204081632653061No Hit
GTTTCAACCCTGTACGTGATTTATTGAAACTCAATAAAAAAGATGGTGAG11.0204081632653061No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA11.0204081632653061No Hit
TCCAAGAGGCGATTGAAGAACCTGAAGTTCAAGGTGGAAGACAATTCTTT11.0204081632653061No Hit
ATCAACGCAGACTACGGGGGCGGTTGAAACAGAGGAATCGAAAGAAAACA11.0204081632653061No Hit
ATTTGTAGCAAACTTCATTGCAGCTGACAGGCTTCCAGCCTGTAGGCCAG11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGAAAACGAAAAAAAAAAAAGAAAAAAATGGA11.0204081632653061No Hit
ACAGATACAATACAGACATTTCTTAAAAGTTACCGTATCATTCACATGTG11.0204081632653061No Hit
GAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACG11.0204081632653061No Hit
GTGTTCGGGAGTTGTTTGCGTTATAGGGTTTTTAAGTGTTGGTATTCTTG11.0204081632653061No Hit
CAAAAAATTAAAAAGAAATAATTAAAGTAAATTTAACAAGTGAAGAACGA11.0204081632653061No Hit
TATCAACGCAGAGTACGGGGATATGTTGGCTTGCCAGTTGTTATTTTTAC11.0204081632653061No Hit
AAAAAACACCAACACCAAAAGCAAAAAAACCGCAACGCACACCACACAAA11.0204081632653061No Hit
ATATTAGCCCTTTGTCAGATGAGTAGGTTGCAAAAATTTTCTCCCATGTT11.0204081632653061No Hit
ACGCAGAGTCGAGCTTTTTTTTTTTTTTTATTTTTTTTTTTGAAAGTAAC11.0204081632653061No Hit
ATACAGGACTGTGCTTTATATATGCGTAGACTCAGGGTGCTATCTGTCTC11.0204081632653061No Hit
ACAGGTATAAACGCAGAGTACGGGATGGACGATAAGAAAAGCACGAAAAA11.0204081632653061No Hit
ATTCAGAAATCTCCGGCTCACAGGATATTTGCCGCCACCCCTAAGCTTAT11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
GAGTACGGGATTCCATTCCATTCCATTCCAATCAAGTTGATTCCTTTCAA11.0204081632653061No Hit
GCAGCCATGATGTGCAGTTCAATGCCAGTTCAATGCGGCATTTAAGATCA11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCAAGAGACAAGAGGCAATCGCGGATGCCGG11.0204081632653061No Hit
TTCCCCACAGTGATTCGGCACATAAAACAGGTGCTGAACTGTAGCAGTTG11.0204081632653061No Hit
GGAAACAAAACAACACCACCGAAAAACAAAAAACACAAAACCCCCAAACA11.0204081632653061No Hit
CCTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGA11.0204081632653061No Hit
GCCATAGTGAGATTTCTTCACGGTCCAGCATCGTGAGCAATTGATCTTTT11.0204081632653061No Hit
TCACTGGGCAGGCGGTGCCTCTAATAATGGAGATGCTAGAGGTGATGTTT11.0204081632653061No Hit
CACTTTCTAGTTTATCTATTTTAGTCGCTAGTCACGCTTTTGCTGATAAC11.0204081632653061No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA11.0204081632653061No Hit
GACATGCCACTCCGAATTCATGTGCTACTTGGCCTAGCTATCACTACACT11.0204081632653061No Hit
CGGGAGAAGAGGGAGGAGATAGTGAGGCCCATGGCGGTAGTGGCACTTGT11.0204081632653061No Hit
GTTTATAGATAGTTGGGTGGTTGGTGTAATTGAGTGAGGCATGATTACGA11.0204081632653061No Hit
TGTTCTTATCCGTGGTGGTCGTGTTAAAGACTTACCAGTTGTACGTTACC11.0204081632653061No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGCGCCCACGAG11.0204081632653061No Hit
ATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAA11.0204081632653061No Hit
TCACAGATCCATTTATTCATGCCATGAAGTAAACGGTACTTATACAAGTG11.0204081632653061No Hit
CCCCCACGCCCCTAAAAGACCTAAACAATCAGACTAAAAAAAATAAAACA11.0204081632653061No Hit
GCAGAGTACGGGTACCCCCTCAGCAAAATTGACTCCTCTGTAAATTACAG11.0204081632653061No Hit
CAACAAACCATATAAAAAGTACAAAGGAACCAACAACACACAAAATAAAG11.0204081632653061No Hit
ATAGTGGCTCACGCCTGTAATCCCAGCTACTTATGATTCTGAGGCATGCT11.0204081632653061No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTGGGGGGGG11.0204081632653061No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTATGTTTTTAATAAACA11.0204081632653061No Hit
CAATATCCCTGTTCACTTTGAAGTGAATTTTGACTCTATATTCAGAACCT11.0204081632653061No Hit
CATAAACTTTATCCAATAACAAAGCAAAATAACAAAAAAAAAAAAAAAAT11.0204081632653061No Hit
GGCCAACATGGAGCCGCAGATCCACACAGAGTACTTCCCATCAGGAGGAG11.0204081632653061No Hit
CAAAACGGGTGAAAAACCCGTTCGCTGAAAGACCAAGGGTTCCCGTCCAA11.0204081632653061No Hit
CTCCAGACTCCACAGCCGGGAGCTGAGCCCAGAGGCCAGGAGGTCCCTGG11.0204081632653061No Hit
ACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAAC11.0204081632653061No Hit
CTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG11.0204081632653061No Hit
GTGCAGCAATACACGCAGCAATACATGCATAAGGGTTAGCGCCTGTAGAA11.0204081632653061No Hit
CGTGTGGAAAGTTCCACCCTGGATCAGGTGAAGAAACGTTTTGAGGTCAA11.0204081632653061No Hit
ACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATCTGT11.0204081632653061No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
TCAATGAGTTGCTTATTCCAGCAGTAAAACGCTTACGAGACACTTTACAT11.0204081632653061No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.0204081632653061No Hit
ATATTTTGGTCAGTCATTTAGACTTAGATCATGCAGCTGGTATATCAGAC11.0204081632653061No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GAAAAAACAGTGCCCTCCTACCCAACTCGATCCAAAGAAAGCCAGATACC11.0204081632653061No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTGTATGAAT11.0204081632653061No Hit
ATAAAAAATCAAAAAAAAAATAAAAAAACACAAAACAAAAAAAAAAAAAA11.0204081632653061No Hit
AATATCAACCAAAATAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
ACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCAT11.0204081632653061No Hit
TATATGGTTTGGATTTTTGTCCTCACCCAAATCTCATCTATAAATGTAAT11.0204081632653061No Hit
GCGTCAGATGTGTATAAGAGACAGCAGTGGTATCAACGCAGAGTCGAGTT11.0204081632653061No Hit
AGACAGGGTTTAATCATGTTGGCCAGGATTGTCTAGAACTCCTGATGATC11.0204081632653061No Hit
CGGAAAATCGCCTACAAAAAAAGTATCTTGAGCAAGTTGTGGATGCAAAC11.0204081632653061No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG11.0204081632653061No Hit
CTACTAAAGTGAAACTGAGAACTTCGTACTTTAGTTGTATCCTGAAATCA11.0204081632653061No Hit
CTAATGAAAAGTGCTACAGGTTTAACGGGTTCAGGTTCTCGTGATTGGTA11.0204081632653061No Hit
CAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGGCGTAAAAAAGAACAAAACCTGTCTCTTAT11.0204081632653061No Hit
ATATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAATAAAAAATTGT11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA11.0204081632653061No Hit
CCTTTCATGGCTTGATGAAGCTTCTCTGCAAAGAAAGCTGGTTTGCTTGT11.0204081632653061No Hit
CTGGAACCCTTGGTCTTTCAGCGAACGGGTTTTTCACCCGTTTTGTCGTT11.0204081632653061No Hit
AAAAATCACAGACAAAACCAGACACCACAACAGCTCTCAAGCGCCTTTCT11.0204081632653061No Hit
AGGCTGGAATATGTTGGTGCCACCGTACTCTGCGAAGATACCACCTGACT11.0204081632653061No Hit
CTTTCACCCATGATGGAGTGCAGTGGCGGGATCTCGGCTCACTGCAACCT11.0204081632653061No Hit
CAGCACTGACCGTATCCGTGTTGAAGGTGCTGATGTCTATTACACGGTTG11.0204081632653061No Hit
CCGGCAGAAGGTCCAATTTCTCAGTTGGGTAAAATTAAAGATGGTGATGT11.0204081632653061No Hit
TGGATAAATAGATAAGGACGAAAATATGAAAAAAATTGCATTCGCTATTG11.0204081632653061No Hit
ACACAAGATAGTACAAAATGCATTGCAAATTGATTTTTGAGAAGGTGACC11.0204081632653061No Hit
GAGTCGACTTTTTTTTTTTTTTTTTATTTTTTTTTTTTAAGGTTAAATTG11.0204081632653061No Hit
CCTGACAAGAGCCCAGGCCTCAGAATGCCAGACGGCTGTACGAGCCAAGC11.0204081632653061No Hit
CAAATCGTACTAAGAATTTAGGTGCACAAAGGAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
ACGTCTTGCCTGTGTCCCACTTGTTCGCGGCCTATAGGCTACTGCAGCAC11.0204081632653061No Hit
GGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGG11.0204081632653061No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC50.085.02
CTATACG50.085.03
TATACGT50.085.04
ACGTCCA50.085.07
TACGTCC50.085.06
ATACGTC50.085.05
GTCCACT50.085.09
CCCTATA50.085.01
CGTCCAC50.085.08
Produced by FastQC (version 0.11.5)