FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237372
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA5890.24813373102135047No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5240.22075055187637968No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA4750.20010784759786326No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC4320.18199282139426723No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC3690.15545220160760326No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT3580.1508181251369159No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA3450.14534148930792173No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG3450.14534148930792173No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA3250.13691589572485383No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC3230.136073336366547No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA3220.1356520566873936No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC3220.1356520566873936No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT3190.13438821764993344No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.12469878502940532No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA2940.12385622567109852No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG2920.12301366631279173No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG2660.11206039465480344No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT2530.10658375882580928No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC2480.1044773604300423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2600.045.7764932
GGTATCA6150.045.6266061
GTATCAA13100.044.4629861
GCTCTAT409.2875364E-442.51571
GTGGTAT2950.040.353881
TATCAAC14950.039.8056452
TCAACGC15400.038.082434
ATCAACG15450.037.959193
CAACGCA15500.037.836745
ATATACT450.001660813737.775814
AACGCAG15700.037.3547446
ACGCAGA16500.035.5436067
CGCAGAG16500.035.5436068
GCAGAGT16950.034.5999689
CGTGTGC550.00444210230.9074848
CCCTCAC600.00679659828.3318587
ATACTTG600.00679659828.3318586
AACACCG600.00679659828.3318586
CAGAGTA14850.027.18713810-11
GACAGGT1159.108271E-625.8791221
Produced by FastQC (version 0.11.5)