FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325225
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.4138165217227263No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.3289252768397819No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32150.24260031315437003No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.17257446848648342No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.15574713727857534No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.11356562093229451No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14850.11205644324548661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22950.057.960212
GTGGTAT23500.056.6293331
GTATCAA86600.038.982311
GGTATCA45450.036.6705251
TATCAAC96900.035.0421942
ATCAACG103950.032.461183
TCAACGC103850.032.4105954
CAACGCA106300.031.7035735
AACGCAG106800.031.4755656
GTCGAGG15200.029.0775648
GACAGGT7550.028.720251
ACGCAGA122000.027.5540187
CGCAGAG123050.027.3188978
TCGAGGT14900.027.0970529
GCAGAGT127300.026.4746049
CAGAGTA67500.024.99699610-11
AGTCGAG24050.021.9116427
AGAGTAC65100.021.67495310-11
GAGTACG77800.021.55105212-13
GTCTAAC2058.627285E-820.7401141
Produced by FastQC (version 0.11.5)