FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581293
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11510.19800685712712865No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT7080.12179744122155264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18250.037.345722
GTGGTAT18600.036.3631131
GACAGGT2200.026.244331
TTAAACG1057.226845E-926.19609322
GTATCAA59850.025.0363641
AGAGTAC36100.024.8369811
CAGAGTA36500.024.48944510
CGGGATA1553.6379788E-1123.06549317
TATCAAC65150.022.947562
CTAGGAC852.5820651E-522.630483
CGGGACA1254.611502E-822.00092917
GGTATCA32350.021.915943
AACGCAG69050.021.3710216
ATCAACG70150.021.19263
ACCATCG650.00323866921.15291818
TCAACGC70500.021.0094324
AGTACGG43100.020.80313513
CAACGCA71150.020.7788735
GAGTACG43400.020.65933412
TACGGGA23600.020.16148815
Produced by FastQC (version 0.11.5)