FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378284
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.19132486483192143No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.17724939127204553No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.11586871791299906No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.10767011733430847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16500.048.1647572
GTGGTAT16850.046.680191
GTATCAA65900.041.7426151
TATCAAC76150.036.2756462
TCAACGC79400.034.683764
ATCAACG80300.034.4537963
CAACGCA81200.033.9149135
AACGCAG81100.033.9043246
GGTATCA34250.032.2755471
ACGCAGA89300.030.7910527
CGCAGAG90550.030.3659958
GCAGAGT98100.028.1589229
GTCGAGG8000.024.9677588
CAGAGTA63550.023.03803310-11
AGAGTAC62700.020.67302510-11
AGCGTCA3950.020.4422263
GTATTAG7750.019.7497481
GCTTATA3651.8189894E-1218.637511
GAGTACG78600.018.49163212-13
GACAGGT7650.018.340591
Produced by FastQC (version 0.11.5)