FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378284
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21550.1563538428944978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21750.038.5441362
GTGGTAT21900.038.418191
GTATCAA77150.025.980721
TATCAAC84850.023.4533272
CGTTCCG600.00203385922.92029823
CAGAGTA62100.022.89565810
AGAGTAC61900.022.8363511
GGTATCA39500.021.926691
ATCAACG90350.021.840713
TCAACGC90900.021.677544
AACGCAG91350.021.5406686
CAACGCA92250.021.299955
ACGCAGA99750.019.7542677
CGCAGAG101250.019.4150758
CGCGGTA2201.0913936E-1118.74684543
GCAGAGT105950.018.610449
AGTACGG77100.018.0495413
GAGTACG78250.018.03030212
GTCGAGG9100.017.5232498
AACGGCC2607.2759576E-1216.9257637
Produced by FastQC (version 0.11.5)