FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844480
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41220.48811102690413033No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.3627084122773778No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.29118510799545283No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16210.19195244410761653No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.19088669950738918No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.13238916256157637No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.12788935202728305No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.11782398635846912No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.1143899204244032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14700.058.4008752
GTGGTAT15350.054.8366241
GTATCAA57850.041.0057451
TATCAAC65100.037.08112
GGTATCA28900.037.069491
TCAACGC70150.034.3511054
ATCAACG70450.034.2651523
CAACGCA72650.033.169035
AACGCAG75150.032.17686
GTCGAGG10050.031.7161548
GACAGGT3900.031.6116581
TCGAGGT10150.029.7288179
CGCAGAG83800.028.7557268
ACGCAGA84250.028.6508857
CGAGGTA300.00680872228.33141948-49
GCAGAGT87250.027.6186819
CAGAGTA43500.025.45093710-11
CAGTGGT4000.024.4445321
AGTCGAG16100.022.7003297
AGAGTAC42250.022.53235610-11
Produced by FastQC (version 0.11.5)