FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970683
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46260.4765716510951567No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.411977957788485No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.28155432824104265No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.2313834691655257No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.19481128236509757No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.17853408373279434No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13110.13505954055031355No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12430.12805416392375266No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.11692797751686183No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.11126186406890817No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10800.11126186406890817No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.10374138621980607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28500.043.542252
GTGGTAT28650.042.8825231
GTATCAA90550.038.0749661
TATCAAC101400.034.6190382
ATCAACG111650.031.516973
AACGCAG110450.031.4746176
TCAACGC112050.031.3286064
CAACGCA114750.030.7025685
GGTATCA49600.029.7409551
GTCGAGG17950.029.5950058
CGCAGAG125600.027.6781188
ACGCAGA126700.027.5049047
GACAGGT5000.027.2074031
TAGTTCG957.4506126E-526.8411147
TCGAGGT18850.026.8292479
GCAGAGT131000.026.5371889
AGTCGAG25600.025.2334427
GCGTCAG1551.347471E-724.6765084
CAGAGTA65500.024.10531210-11
AGCGTCA1703.287023E-722.4991663
Produced by FastQC (version 0.11.5)