FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685672
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.4149214201542429No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.2789963714429057No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.18799075943016486No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10610.15473870888704802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8300.055.8140641
TGGTATC8950.051.7567562
GTATCAA28300.038.595971
TATCAAC30550.036.1681022
ATCAACG31800.034.74643
TCAACGC31950.034.583274
GGTATCA15650.033.9461821
CAACGCA32700.033.920045
AACGCAG34000.032.6230936
ACGCAGA37200.029.7004017
CGCAGAG37500.029.6894368
GCAGAGT40250.027.555399
CGGTAGA808.607154E-426.562891
CAGAGTA23950.024.84016210-11
CCAACGT900.001710944323.6097343
GTACTGG2352.7284841E-1123.5109831
GAGTACG26000.022.96333112-13
AGAGTAC22150.022.44626410-11
GTACGGG28050.020.98209414-15
AGTACGG25000.019.97237812-13
Produced by FastQC (version 0.11.5)