FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267264
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.2589200191570881No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT6780.25368175287356326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC4720.1766044061302682No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.15939295977011494No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.10326867816091954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2450.052.0392152
CTACTAG250.00779069650.998433
GTGGTAT2900.043.9641651
GTATCAA10200.040.415421
GGTATCA5200.039.229561
TATCAAC11650.036.8443572
TAGACGT500.002785598233.998954
ATCAACG12850.033.4036373
TCAACGC12900.033.274174
CAACGCA12950.033.14575
AACGCAG13750.031.2172186
GTACTAC703.9359357E-430.3562051
CGCAGAG15250.028.4253548
ACGCAGA15350.028.2401717
ACAGGTA1106.473416E-627.0446221
GCAGAGT16300.026.5942739
GTATAAG1051.4668147E-424.2849663
GACAGGT1251.7446537E-523.7992671
CAGAGTA10900.023.39377410-11
TTGAGTA1102.010711E-423.1811053
Produced by FastQC (version 0.11.5)