FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813846
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.3383932586754742No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22840.28064277516876657No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.20433841291841454No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.14449908213593235No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.13086013815881628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11800.053.3029632
GTGGTAT11600.053.1457441
GTATCAA43700.041.349091
TATCAAC47150.038.6676032
TCAACGC50600.035.9471864
ATCAACG50850.035.770463
CAACGCA51500.035.3189855
GGTATCA22200.034.089881
AACGCAG53950.033.715066
CGCAGAG59350.030.7906888
ACGCAGA60400.030.325787
GCAGAGT62900.029.1204649
GACAGGT5600.025.8136481
CAGAGTA32850.024.70982610-11
GTCGAGG8600.024.2141538
TCGAGGT8800.021.732099
AGTCGAG11400.021.6219487
AGAGTAC30650.021.56113210-11
GAGTACG37600.021.4752112-13
TACTACG1050.00418658420.237297
Produced by FastQC (version 0.11.5)