FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716379
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT21830.30472696715007No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT19240.26857292020006174No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC17970.2508448740122198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16630.2321396914203236No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC16200.226137282081133No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA15290.2134345088284274No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT14710.20533823576626337No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA14540.20296519021356013No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT13900.19403137166220674No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT13760.19207709885409818No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA13560.18928528055680024No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA13520.18872691689734064No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG13000.181468189324366No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC12240.17085927979463383No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA12170.16988214339057958No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC12010.1676486887527412No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA11860.16555482502976776No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTA11020.15382918818111643No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCC10960.15299164269192705No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATT10830.1511769607986834No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGAT9980.13931173303516714No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGATA9940.13875336937570754No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCT9920.13847418754597773No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTT9660.13484482375949045No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTG9560.13344891461084146No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGA9550.13330932369597656No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAAC9390.13107586905813823No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTA9230.12884241442029987No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAG9000.12563182337840723No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAACGGG8990.12549223246354235No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGAAATCAAT8870.12381714148516358No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCT8710.12158368684732523No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCCCCT8580.11976900495408156No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGG7660.10692664078651105No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCA7370.10287850425542903No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGA7310.10204095876623966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29950.037.4592481
TGGTATC30450.036.9267232
CCGGATG350.004097515731.43028624
GACAGGT2100.030.1164861
CGGGAGC1201.8189894E-1229.79537417
ACACGTG400.00787713727.4957398
CGGGATC5050.026.68649117
GTATCAA111300.026.5835441
AGAGTAC69350.026.13519111
CAGAGTA69800.026.0849110
GGGATCG3450.025.50863818
TATCAAC120900.024.4220872
TGAGTAC801.6116732E-524.0537344
GGGGTAC1052.095112E-723.57271618
GTGTCGA1052.0965308E-723.57106855
GCTTGTA2100.023.5694241
ACAGGAG1653.6379788E-1223.3313481
GACAGAT1302.746674E-923.267251
TCGACAC3450.023.11720322
ACGGGAT13550.022.93803416
Produced by FastQC (version 0.11.5)