FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065694
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.2876998462973424No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT29950.2810375210895435No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.2017464675601064No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.1338095175538194No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC10860.10190542500943048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10350.047.2183882
GTGGTAT11750.043.05321
GTATCAA38550.041.683461
TATCAAC44150.036.865642
TCAACGC46550.034.7807244
ATCAACG47000.034.4493333
CAACGCA47400.034.2466665
GGTATCA19900.033.1112061
AACGCAG49800.032.6815576
ACGCAGA52900.030.846717
CGCAGAG53000.030.7899538
GCAGAGT58700.027.8001299
ACGTACG408.615598E-426.56158834-35
CAGAGTA39250.024.25280810-11
CGTATAT900.001708522323.6169531
GAGTACG43200.021.83850712-13
AGAGTAC38450.021.33116710-11
TACCGTC1050.00418956120.2355047
TACGGGT3800.020.12994614-15
GTACGGG46700.020.0197914-15
Produced by FastQC (version 0.11.5)