FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784909
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22690.12712132663345863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20700.038.243482
GTGGTAT21150.037.8302841
GTATCAA71350.026.0115831
AGAGTAC59600.023.85672611
TATCAAC78150.023.7767772
CAGAGTA62300.022.95459710
GGTATCA36200.022.7784213
ATCAACG81200.022.7470053
TCAACGC80200.022.7221894
CAACGCA81500.022.3597475
AACGCAG83100.022.128316
ACGCAGA90750.020.3252327
CGCAGAG91850.019.9948338
TCGCGGC700.00498049619.6405359
TATTCCG700.004991808419.6328325
GCAGAGT98500.018.6475419
AGTACGG75700.018.42005213
ATCGTCG750.007407719318.3357946
GTCGCGG909.835603E-418.3285988
GAGTACG77250.018.12166212
Produced by FastQC (version 0.11.5)