FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872609
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55010.29376127103949623No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.2815857448084464No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.1773995532436296No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA32600.17408866453167748No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.15491755086085776No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA25700.1372416772535003No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT25170.13441140141908964No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC25040.13371718281819642No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT24620.13147432272300302No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG23220.12399812240569173No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA21880.11684233067340806No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA21270.11358484339229384No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.11214300475966954No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.10498721302738587No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC19360.10338517010224772No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGA18760.10018108425197145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC38500.041.3940122
GTATCAA138500.040.08771
GTGGTAT43250.036.762351
AACGCAG157250.035.727926
TATCAAC158400.035.3880422
TCAACGC163050.034.3527574
ATCAACG167450.033.55163
CAACGCA167550.033.4301225
ACGCAGA174000.032.2885977
CGCAGAG175600.031.9701948
GTCGAGG15900.030.2028758
GACAGGT7200.030.1130981
GCAGAGT190050.029.5409979
GGTATCA76600.027.3057481
ACAGGTA9500.024.1650621
CAGAGTA116500.023.62131110-11
GCTTGTA11000.023.1886941
CGGGCCA1759.8265446E-821.8560374
AGAGTAC113650.021.16591810-11
CAGGTAT9700.021.0371671
Produced by FastQC (version 0.11.5)