FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872609
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA30920.16511722415090388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27650.1476549562668982No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT23260.1242117281290435No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT22850.12202226946468804No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC21950.11721614068927362No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC20180.10776408743095862No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT20120.107443678845931No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA19160.10231714148548895No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG19030.10162292288459576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC42650.034.6705472
GTGGTAT46400.032.1157461
GTATCAA157200.026.8992671
GACAGGT5200.025.9077341
GCTTGTA9050.025.5192221
AGAGTAC124600.024.32425311
CAGAGTA126300.024.2575110
TATCAAC175150.024.1661832
AACGCAG180700.023.3783136
TCAACGC183950.022.8301874
TCCGGAC1453.947207E-1022.748127
ATCAACG185400.022.6812823
CAACGCA186700.022.538075
GCACCGC4000.022.00160410
CGATCGG1658.367351E-1121.66882716
ACGCAGA200400.021.0406887
CGCAGAG201400.020.9259198
CGGGCCG1459.6915755E-920.86358817
CCCTTGC1851.8189894E-1120.81566453
CCGGACT1601.3460522E-920.6187888
Produced by FastQC (version 0.11.5)