FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975512
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15200.15581561272439498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19950.038.4372832
GTGGTAT20550.037.3303381
GTATCAA64550.026.0264241
AGAGTAC48550.024.86887711
CAGAGTA49500.024.39159810
TATCAAC72500.023.1250782
GGTATCA33800.023.0123583
GCTTGTC2700.022.4041481
ATCAACG76950.021.8234793
TCAACGC77350.021.6750894
AACGCAG77900.021.6267936
CAACGCA78050.021.5159075
TAGTGCG700.004969361219.64705348
ACGCAGA86800.019.4102947
CGCAGAG86500.019.356478
GCTTGTA3600.019.0944421
AGTACGG62750.018.93534313
GAGTACG64200.018.54956612
GCAGAGT90150.018.546079
CCGGATT1252.4787536E-517.60285813
Produced by FastQC (version 0.11.5)