FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077576
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.29872602953295174No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.26271928847710047No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.17242403320044247No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.15237904333429847No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA15410.14300615455429594No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA12650.11739311194755636No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.11590829788339756No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT12420.11525869173032807No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC11970.11108265217488139No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.10709221437745459No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT11220.10412258624913695No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA10890.10106015724180939No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10850.10068895372576968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24400.038.664742
GTGGTAT26250.036.433921
GTATCAA77450.035.838061
GACAGGT4100.033.1756061
GTCGAGG11300.031.9692738
AACGCAG88500.031.5016046
TATCAAC89200.031.3958682
TCAACGC91800.030.5080764
ATCAACG93650.029.8600253
CAACGCA94950.029.5407185
ACGCAGA99600.028.0348557
CGCAGAG100050.027.866288
GGTATCA46900.026.8269651
GCAGAGT108300.025.8219919
GCTTGTA5750.023.655651
CAGAGTA63400.022.92604310-11
TCGAGGT15900.022.1857059
GACAGGC2759.094947E-1221.6395421
GTACGGC1403.8253074E-521.2491763
AGTCGAG21100.020.3437567
Produced by FastQC (version 0.11.5)