FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867127
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.23903033912529786No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43580.23340672594847592No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.15017725093151135No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22580.12093446241203733No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.11938127401081983No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA19390.10384938999864499No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT18800.10068945497547838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24450.051.4484062
GTGGTAT26450.048.062061
GTATCAA95900.041.142081
TATCAAC110500.035.882152
TCAACGC114150.034.6603434
ATCAACG114550.034.539313
GGTATCA51550.034.3100621
AACGCAG116050.034.0562556
CAACGCA116400.033.9538545
ACGCAGA130550.030.3387817
CGCAGAG131250.030.1454058
GCAGAGT142300.027.8052719
GTCGAGG12650.026.2044358
CAGAGTA86800.023.03896710-11
AGAGTAC83700.020.61691310-11
TCGAGGT16850.019.168859
GAGTACG105600.018.85708612-13
AGTCGAG22700.018.533977
TACGAAG3007.421477E-1018.4154012
GACAGGT9250.017.925851
Produced by FastQC (version 0.11.5)