FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867127
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30130.16137091906442358No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT20740.11107974979741603No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA19810.10609883526937373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC31150.039.966892
GTGGTAT31850.039.4537241
CGTACGA701.6073136E-731.43411835
GTATCAA116500.026.9302881
AGAGTAC88050.024.61271911
ACGTACG901.4459856E-624.44810334
TATCAAC128200.024.3743272
CAGAGTA91400.023.86106110
ATCAACG135950.022.9241923
TCAACGC136400.022.8284134
CAACGCA138750.022.4411685
AACGCAG139600.022.3450986
GGTATCA59600.022.052761
ACGCAGA154500.020.2305877
CGCAGAG155850.020.0045768
AGTACGG108850.019.65684913
CGGGATC5100.019.41310717
GAGTACG111450.019.34633612
GCAGAGT163550.019.1172819
GACAGGT6700.018.4679621
Produced by FastQC (version 0.11.5)