FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335759
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.3174896279772098No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.2260549977811466No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA6060.18048659901893918No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.15963831200355016No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT4410.1313442081969508No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC4410.1313442081969508No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA3650.10870892515167128No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT3610.10751759446507762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4000.047.810492
GTGGTAT4400.043.502961
GTATCAA16150.042.6679151
GGTATCA7500.041.4019241
TATCAAC18350.038.213652
ATCAACG18500.037.668483
TCAACGC18650.037.3655174
CAACGCA19300.036.1070945
AACGCAG19550.035.6453636
CTCGTAG500.002774572234.028981
ACGCAGA21800.032.161297
CGCAGAG21900.032.0144358
TATTAGG1201.1184966E-831.873662
GTATTAG1657.2759576E-1230.9354361
GCAGAGT23150.030.1067249
ATTAGGC1152.7437272E-729.559573
AGTACTG1255.6896897E-727.1948075
TAATGGA957.4441574E-526.8369774
CAGAGTA16050.025.68427810-11
GTCTATC850.001218676225.0213071
Produced by FastQC (version 0.11.5)