FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335759
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5260.15665998528706604No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT4530.13491820025673176No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT3800.1131764152263975No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC3630.10811325980837447No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT3380.10066744301716408No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC300.001911123936.7156071
AGCGTCC300.001929397836.6445247
GTATCAA16300.033.2805561
TGGTATC3200.030.9556982
GTGGTAT3350.029.5916841
TATCAAC18850.029.3408582
TCAACGC19150.027.9898724
ATCAACG19400.027.916713
AACGCAG19250.027.8403226
CAACGCA19550.027.6942675
CTGCCGA2600.027.51617855
TGCCGAC1203.6379788E-1127.51617655
AGAGTAC19300.027.49158111
CAGAGTA19750.027.27872510
CGCAGAG19900.027.0690738
ACGCAGA20150.026.5968347
GCAGAGT20700.025.894019
ACGGGGT2800.025.53931216
GGTATCA6500.025.4184971
ACTATCG651.0889783E-425.37684611
Produced by FastQC (version 0.11.5)