FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117100
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20640.18476412138573092No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA18180.16274281622057113No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13800.12353415092650613No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA13260.11870020589025154No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13180.11798406588488049No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT12800.11458240085936802No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12490.1118073583385552No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG11750.10518306328887299No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA11330.10142332826067496No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT11280.10097574075731805No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA11270.10088622325664666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC36950.033.0283282
GTGGTAT38100.031.9807451
GCTTATA1500.029.3385431
TGCGACG400.0078713827.50118655
GACAGGT3250.027.0817321
GTATCAA118600.025.3249021
GCTTGTA5200.024.3312441
AGAGTAC85950.024.03169411
CAGAGTA87300.023.81759510
GCTTGTC2200.023.7542171
GACACGC600.002032333322.9227935
TATCAAC132700.022.743092
AACGCAG134350.022.5201076
TTGTACG1103.3095967E-722.4838473
TCAACGC140950.021.4656034
ATCAACG141600.021.4058783
CAACGCA142950.021.1835525
CGGGCCA804.4637418E-420.6101936
GGTATCA63850.020.1851863
ACGCAGA150000.020.1530917
Produced by FastQC (version 0.11.5)