FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802578
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.3349207179862892No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.2895668707589792No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.19262925223467378No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.14403584449112736No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.13406796597963064No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.10241995170562862No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA8150.10154776233587265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12950.057.132391
TGGTATC13150.055.5890272
GTATCAA43900.036.903141
GGTATCA25800.033.291591
TATCAAC51450.031.719932
ATCAACG52550.031.0559543
TCAACGC52900.030.8504834
GTCGAGG8500.029.9998258
CAACGCA54900.029.8814285
AACGCAG55900.029.3468766
TCGAGGT8200.029.0242219
ACGCAGA62450.026.2688627
ACACCGT1159.170333E-625.8694136
CGCAGAG63500.025.8344948
AGTCGAG11950.024.8952527
GCAGAGT66600.024.8234279
CAGAGTA35050.024.31155810-11
GTCTAGC900.00170571923.6227471
GACAGGT4950.022.3342321
ACCGTCT1152.7227943E-422.1737828
Produced by FastQC (version 0.11.5)