FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802578
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21640.26963111373598575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13750.041.1986431
TGGTATC14050.039.518822
GTATCAA52350.026.8424781
AGAGTAC31500.025.84364311
CAGAGTA33100.024.92675810
TATCAAC57850.024.327392
GGTATCA26600.023.2605131
ATCAACG60550.023.1957633
TCAACGC60850.023.0814024
TACGCGA600.002034647922.91734329
ACGCGAG600.002036123522.91448630
CAACGCA61700.022.8079725
AACGCAG62600.022.6556876
TAGAACG852.5734398E-522.64207844
GTCTTAC1103.2854223E-722.499261
AGTACGG35900.022.37113813
GAGTACG36900.021.98848512
ACGGGTC903.9889455E-521.39218516
ACGCAGA70250.020.2302027
ACGGGAT9450.020.0824616
Produced by FastQC (version 0.11.5)