FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357092
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.45693291243335016No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.3209067624007805No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.22386102047613574No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT13920.10257226481329194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15700.056.8473322
GTGGTAT16100.056.773681
GTATCAA57850.037.921091
GGTATCA31300.037.6244281
TATCAAC63050.034.579822
TCAACGC66300.032.8194164
ATCAACG66300.032.7553183
CAACGCA67750.032.117015
AACGCAG71700.030.4662086
CGCAGAG78300.027.896128
ACGCAGA79750.027.3356327
GCAGAGT84150.025.9577739
CAGAGTA45450.025.94702110-11
GAGTACG53050.022.02955612-13
AGAGTAC44500.021.91710-11
GTACGGG55850.020.62075414-15
AGTACGG48950.019.7509112-13
TATATCG1306.232672E-419.614735
CTTAGAC2252.3118264E-718.8882583
GACAGGT7350.018.5096051
Produced by FastQC (version 0.11.5)